evs secretion inhibitor gw4869 Search Results


evs secretion inhibitor gw4869  (MedChemExpress)
96
MedChemExpress evs secretion inhibitor gw4869
Evs Secretion Inhibitor Gw4869, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
evs secretion inhibitor gw4869 - by Bioz Stars, 2026-02
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gw4869  (Millipore)
90
Millipore gw4869
Extracellular vesicles from neuronal cells are robustly taken up by phagocytic cells. A . Western blot of the EV enriched markers (TSG‐101, Flotillin‐1, Syntenin‐1) and intracellular contamination markers (GRP78, synaptophysin) in cortical neuron derived EVs and cell lysates. B . Size distribution of the cortical neuron and N2A cell derived EVs by NTA (n = 3). C . Particle concentrations of the EVs from N2A cells after <t>GW4869</t> exposure quantified by NTA and EVQuant. Mann‐Whitney U test for unequal variances was performed. D . Particle concentrations based on specific sized particles upon GW4869 exposure measured by NTA. Comparison between control and treatment was performed with T‐test except for the 70–90 nm bin where Mann–Whitney U test for unequal variances was used. E . Uptake of PKH26 stained cortical neuron EVs by different cell lines quantified by confocal microscopy (n = 3). F . Comparison of the uptake kinetics of CXN and N2A cell EVs in microglial and endothelial cell lines (n = 3). Statistical significance for both EV types was found with Two‐way ANOVA test, p < 0.001 for both time and cell type. G . Representative images of the EV uptake in C166 (upper panel) and BV2 (lower panel) cells at 3 h. Green = lipid membranes of the cells stained with PKH67, blue = nuclei stained with Hoechst, red = EVs stained with PKH26. Scale bar = 50 μm. The dots in C and D correspond to technical replicates (n = 4–6). The data is represented as mean ± SD and the significance is stated as *** p < 0.001, ** p < 0.01, * p < 0.05
Gw4869, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gw4869/product/Millipore
Average 90 stars, based on 1 article reviews
gw4869 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

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Extracellular vesicles from neuronal cells are robustly taken up by phagocytic cells. A . Western blot of the EV enriched markers (TSG‐101, Flotillin‐1, Syntenin‐1) and intracellular contamination markers (GRP78, synaptophysin) in cortical neuron derived EVs and cell lysates. B . Size distribution of the cortical neuron and N2A cell derived EVs by NTA (n = 3). C . Particle concentrations of the EVs from N2A cells after GW4869 exposure quantified by NTA and EVQuant. Mann‐Whitney U test for unequal variances was performed. D . Particle concentrations based on specific sized particles upon GW4869 exposure measured by NTA. Comparison between control and treatment was performed with T‐test except for the 70–90 nm bin where Mann–Whitney U test for unequal variances was used. E . Uptake of PKH26 stained cortical neuron EVs by different cell lines quantified by confocal microscopy (n = 3). F . Comparison of the uptake kinetics of CXN and N2A cell EVs in microglial and endothelial cell lines (n = 3). Statistical significance for both EV types was found with Two‐way ANOVA test, p < 0.001 for both time and cell type. G . Representative images of the EV uptake in C166 (upper panel) and BV2 (lower panel) cells at 3 h. Green = lipid membranes of the cells stained with PKH67, blue = nuclei stained with Hoechst, red = EVs stained with PKH26. Scale bar = 50 μm. The dots in C and D correspond to technical replicates (n = 4–6). The data is represented as mean ± SD and the significance is stated as *** p < 0.001, ** p < 0.01, * p < 0.05

Journal: Journal of Extracellular Vesicles

Article Title: Dynamic release of neuronal extracellular vesicles containing miR‐21a‐5p is induced by hypoxia

doi: 10.1002/jev2.12297

Figure Lengend Snippet: Extracellular vesicles from neuronal cells are robustly taken up by phagocytic cells. A . Western blot of the EV enriched markers (TSG‐101, Flotillin‐1, Syntenin‐1) and intracellular contamination markers (GRP78, synaptophysin) in cortical neuron derived EVs and cell lysates. B . Size distribution of the cortical neuron and N2A cell derived EVs by NTA (n = 3). C . Particle concentrations of the EVs from N2A cells after GW4869 exposure quantified by NTA and EVQuant. Mann‐Whitney U test for unequal variances was performed. D . Particle concentrations based on specific sized particles upon GW4869 exposure measured by NTA. Comparison between control and treatment was performed with T‐test except for the 70–90 nm bin where Mann–Whitney U test for unequal variances was used. E . Uptake of PKH26 stained cortical neuron EVs by different cell lines quantified by confocal microscopy (n = 3). F . Comparison of the uptake kinetics of CXN and N2A cell EVs in microglial and endothelial cell lines (n = 3). Statistical significance for both EV types was found with Two‐way ANOVA test, p < 0.001 for both time and cell type. G . Representative images of the EV uptake in C166 (upper panel) and BV2 (lower panel) cells at 3 h. Green = lipid membranes of the cells stained with PKH67, blue = nuclei stained with Hoechst, red = EVs stained with PKH26. Scale bar = 50 μm. The dots in C and D correspond to technical replicates (n = 4–6). The data is represented as mean ± SD and the significance is stated as *** p < 0.001, ** p < 0.01, * p < 0.05

Article Snippet: To inhibit the release of EVs, cells were treated with 5 μM GW4869 (Sigma–Aldrich, St. Louis, MO, USA), or control containing the same concentration of DMSO, for 24 h before the medium was collected for the EV isolation.

Techniques: Western Blot, Derivative Assay, MANN-WHITNEY, Staining, Confocal Microscopy